Construct: shRNA TRCN0000113991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010380.2-1165s1c1
DNA Barcode:
CCCTCAGTTCTCTTTAGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H2-D1 (14964)
Gene Description:
histocompatibility 2, D region locus 1
Transcript:
RefSeq NM_010380.2 (NON-CURRENT)
Match location:
Position 1165 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGTTCTCTTTAGTCAA
Hairpin Sequence:
5'-CCGG-CCCTCAGTTCTCTTTAGTCAA-CTCGAG-TTGACTAAAGAGAACTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGTTCTCTTTAGTCAACTCGAGTTGACTAAAGAGAACTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGTTCTCTTTAGTCAACTCGAGTTGACTAAAGAGAACTGAGGG-3'

Other clones with same target sequence:

(none)