Construct: shRNA TRCN0000114044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019909.1-903s1c1
DNA Barcode:
TGACTCTTACATGGTGATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H2-K1 (14972)
Gene Description:
histocompatibility 2, K1, K region
Transcript:
RefSeq NM_019909.1 (NON-CURRENT)
Match location:
Position 903 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACTCTTACATGGTGATCAT
Hairpin Sequence:
5'-CCGG-TGACTCTTACATGGTGATCAT-CTCGAG-ATGATCACCATGTAAGAGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACTCTTACATGGTGATCATCTCGAGATGATCACCATGTAAGAGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACTCTTACATGGTGATCATCTCGAGATGATCACCATGTAAGAGTCA-3'

Other clones with same target sequence:

(none)