Construct: shRNA TRCN0000114054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008208.2-463s1c1
DNA Barcode:
CGCGATTACATTGCCCTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H2-T3 (15043)
Gene Description:
histocompatibility 2, T region locus 3
Transcript:
RefSeq NM_008208.2 (NON-CURRENT)
Match location:
Position 463 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGATTACATTGCCCTCAAT
Hairpin Sequence:
5'-CCGG-CGCGATTACATTGCCCTCAAT-CTCGAG-ATTGAGGGCAATGTAATCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGATTACATTGCCCTCAATCTCGAGATTGAGGGCAATGTAATCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGATTACATTGCCCTCAATCTCGAGATTGAGGGCAATGTAATCGCG-3'

Other clones with same target sequence:

(none)