Construct: shRNA TRCN0000114082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015780.1-210s1c1
DNA Barcode:
CTGTTCTTAGTGGGAAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfhr1 (50702)
Gene Description:
complement factor H-related 1
Transcript:
RefSeq NM_015780.1 (NON-CURRENT)
Match location:
Position 210 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTCTTAGTGGGAAGATTT
Hairpin Sequence:
5'-CCGG-CTGTTCTTAGTGGGAAGATTT-CTCGAG-AAATCTTCCCACTAAGAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTCTTAGTGGGAAGATTTCTCGAGAAATCTTCCCACTAAGAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTCTTAGTGGGAAGATTTCTCGAGAAATCTTCCCACTAAGAACAG-3'

Other clones with same target sequence:

(none)