Construct: shRNA TRCN0000114116
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013773.1-652s1c1
- DNA Barcode:
- CCTGACCTTATAGATGGCTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tcl1b1 (27379)
- Gene Description:
- T cell leukemia/lymphoma 1B, 1
- Transcript:
- RefSeq NM_013773.1 (NON-CURRENT)
- Match location:
- Position 652 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000114116)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGACCTTATAGATGGCTTT
- Hairpin Sequence:
-
5'-CCGG-CCTGACCTTATAGATGGCTTT-CTCGAG-AAAGCCATCTATAAGGTCAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGACCTTATAGATGGCTTTCTCGAGAAAGCCATCTATAAGGTCAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGACCTTATAGATGGCTTTCTCGAGAAAGCCATCTATAAGGTCAGG-3'
Other clones with same target sequence:
(none)
