Construct: shRNA TRCN0000114142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182939.1-458s1c1
DNA Barcode:
CCCAATCCTAATGTTGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp4r2 (232314)
Gene Description:
protein phosphatase 4, regulatory subunit 2
Transcript:
RefSeq NM_182939.1 (NON-CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATCCTAATGTTGAATAT
Hairpin Sequence:
5'-CCGG-CCCAATCCTAATGTTGAATAT-CTCGAG-ATATTCAACATTAGGATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATCCTAATGTTGAATATCTCGAGATATTCAACATTAGGATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATCCTAATGTTGAATATCTCGAGATATTCAACATTAGGATTGGG-3'

Other clones with same target sequence:

  1. TRCN0000334789