Construct: shRNA TRCN0000114186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026314.2-1601s1c1
DNA Barcode:
GCCAGGATACTACTGAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dyx1c1 (67685)
Gene Description:
dyslexia susceptibility 1 candidate 1
Transcript:
RefSeq NM_026314.2 (NON-CURRENT)
Match location:
Position 1601 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGATACTACTGAGGAAT
Hairpin Sequence:
5'-CCGG-GCCAGGATACTACTGAGGAAT-CTCGAG-ATTCCTCAGTAGTATCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGATACTACTGAGGAATCTCGAGATTCCTCAGTAGTATCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGATACTACTGAGGAATCTCGAGATTCCTCAGTAGTATCCTGGC-3'

Other clones with same target sequence:

(none)