Construct: shRNA TRCN0000114280

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026542.1-793s1c1
DNA Barcode:
GTACTGGTTCAACTACGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a39 (68066)
Gene Description:
solute carrier family 25, member 39
Transcript:
RefSeq NM_026542.1 (NON-CURRENT)
Match location:
Position 793 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114280)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTGGTTCAACTACGAGTT
Hairpin Sequence:
5'-CCGG-GTACTGGTTCAACTACGAGTT-CTCGAG-AACTCGTAGTTGAACCAGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTGGTTCAACTACGAGTTCTCGAGAACTCGTAGTTGAACCAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTGGTTCAACTACGAGTTCTCGAGAACTCGTAGTTGAACCAGTAC-3'

Other clones with same target sequence:

  1. TRCN0000317682