Construct: shRNA TRCN0000114305

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146090.2-840s1c1
DNA Barcode:
CCTACAGGACTCTCACCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zadh2 (225791)
Gene Description:
zinc binding alcohol dehydrogenase, domain containing 2
Transcript:
RefSeq NM_146090.2 (NON-CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114305)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACAGGACTCTCACCAATA
Hairpin Sequence:
5'-CCGG-CCTACAGGACTCTCACCAATA-CTCGAG-TATTGGTGAGAGTCCTGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACAGGACTCTCACCAATACTCGAGTATTGGTGAGAGTCCTGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACAGGACTCTCACCAATACTCGAGTATTGGTGAGAGTCCTGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000324092