Construct: shRNA TRCN0000114354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012019.2-1210s1c1
DNA Barcode:
CGGTGGCAGGTTACTCATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aifm1 (26926)
Gene Description:
apoptosis-inducing factor, mitochondrion-associated 1
Transcript:
RefSeq NM_012019.2 (NON-CURRENT)
Match location:
Position 1210 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGGCAGGTTACTCATTAA
Hairpin Sequence:
5'-CCGG-CGGTGGCAGGTTACTCATTAA-CTCGAG-TTAATGAGTAACCTGCCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGGCAGGTTACTCATTAACTCGAGTTAATGAGTAACCTGCCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGGCAGGTTACTCATTAACTCGAGTTAATGAGTAACCTGCCACCG-3'

Other clones with same target sequence:

  1. TRCN0000345279