Construct: shRNA TRCN0000114420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146041.1-489s1c1
DNA Barcode:
GCCTTATAAATTCTGTGAAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gmds (218138)
Gene Description:
GDP-mannose 4, 6-dehydratase
Transcript:
RefSeq NM_146041.1 (NON-CURRENT)
Match location:
Position 489 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTATAAATTCTGTGAAGT
Hairpin Sequence:
5'-CCGG-GCCTTATAAATTCTGTGAAGT-CTCGAG-ACTTCACAGAATTTATAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTATAAATTCTGTGAAGTCTCGAGACTTCACAGAATTTATAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTATAAATTCTGTGAAGTCTCGAGACTTCACAGAATTTATAAGGC-3'

Other clones with same target sequence:

(none)