Construct: shRNA TRCN0000114447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025388.1-1348s1c1
DNA Barcode:
GCCAAGTACAACCAGATCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eno1b (433182)
Gene Description:
enolase 1B, retrotransposed
Transcript:
RefSeq NM_001025388.1 (CURRENT)
Match location:
Position 1348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGTACAACCAGATCCTT
Hairpin Sequence:
5'-CCGG-GCCAAGTACAACCAGATCCTT-CTCGAG-AAGGATCTGGTTGTACTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGTACAACCAGATCCTTCTCGAGAAGGATCTGGTTGTACTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGTACAACCAGATCCTTCTCGAGAAGGATCTGGTTGTACTTGGC-3'

Other clones with same target sequence:

(none)