Construct: shRNA TRCN0000114535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080633.1-968s1c1
DNA Barcode:
CCATACAACCACAGGATGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aco2 (11429)
Gene Description:
aconitase 2, mitochondrial
Transcript:
RefSeq NM_080633.1 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACAACCACAGGATGAAA
Hairpin Sequence:
5'-CCGG-CCATACAACCACAGGATGAAA-CTCGAG-TTTCATCCTGTGGTTGTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACAACCACAGGATGAAACTCGAGTTTCATCCTGTGGTTGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACAACCACAGGATGAAACTCGAGTTTCATCCTGTGGTTGTATGG-3'

Other clones with same target sequence:

  1. TRCN0000326038