Construct: shRNA TRCN0000114551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178689.2-1979s1c1
DNA Barcode:
CGCTCTGAACTCCACTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eno4 (226265)
Gene Description:
enolase 4
Transcript:
RefSeq NM_178689.2 (NON-CURRENT)
Match location:
Position 1979 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCTGAACTCCACTTATAT
Hairpin Sequence:
5'-CCGG-CGCTCTGAACTCCACTTATAT-CTCGAG-ATATAAGTGGAGTTCAGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCTGAACTCCACTTATATCTCGAGATATAAGTGGAGTTCAGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCTGAACTCCACTTATATCTCGAGATATAAGTGGAGTTCAGAGCG-3'

Other clones with same target sequence:

(none)