Construct: shRNA TRCN0000114567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026425.1-191s1c1
DNA Barcode:
GCGGAGAACTAATGGGTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Naa20 (67877)
Gene Description:
N(alpha)-acetyltransferase 20, NatB catalytic subunit
Transcript:
RefSeq NM_026425.1 (NON-CURRENT)
Match location:
Position 191 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGAACTAATGGGTTATA
Hairpin Sequence:
5'-CCGG-GCGGAGAACTAATGGGTTATA-CTCGAG-TATAACCCATTAGTTCTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGAACTAATGGGTTATACTCGAGTATAACCCATTAGTTCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGAACTAATGGGTTATACTCGAGTATAACCCATTAGTTCTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000325971