Construct: shRNA TRCN0000114571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178604.2-1448s1c1
DNA Barcode:
GCTTTCTGCTTGGAATGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D18Wsu98e (28062)
Gene Description:
DNA segment, Chr 18, Wayne State University 98, expressed
Transcript:
RefSeq NM_178604.2 (NON-CURRENT)
Match location:
Position 1448 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCTGCTTGGAATGCCAA
Hairpin Sequence:
5'-CCGG-GCTTTCTGCTTGGAATGCCAA-CTCGAG-TTGGCATTCCAAGCAGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCTGCTTGGAATGCCAACTCGAGTTGGCATTCCAAGCAGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCTGCTTGGAATGCCAACTCGAGTTGGCATTCCAAGCAGAAAGC-3'

Other clones with same target sequence:

(none)