Construct: shRNA TRCN0000114596

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029331.2-2301s1c1
DNA Barcode:
GCCGGGAAGATGTATGTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nat8f4 (75541)
Gene Description:
N-acetyltransferase 8 (GCN5-related) family member 4
Transcript:
RefSeq NM_029331.2 (NON-CURRENT)
Match location:
Position 2301 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114596)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGGAAGATGTATGTTGTA
Hairpin Sequence:
5'-CCGG-GCCGGGAAGATGTATGTTGTA-CTCGAG-TACAACATACATCTTCCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGGAAGATGTATGTTGTACTCGAGTACAACATACATCTTCCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGGAAGATGTATGTTGTACTCGAGTACAACATACATCTTCCCGGC-3'

Other clones with same target sequence:

(none)