Construct: shRNA TRCN0000114699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009167.1-139s1c1
DNA Barcode:
CTGCGCTCAATGAGGTCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shc3 (20418)
Gene Description:
src homology 2 domain-containing transforming protein C3
Transcript:
RefSeq NM_009167.1 (NON-CURRENT)
Match location:
Position 139 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGCTCAATGAGGTCTCTT
Hairpin Sequence:
5'-CCGG-CTGCGCTCAATGAGGTCTCTT-CTCGAG-AAGAGACCTCATTGAGCGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGCTCAATGAGGTCTCTTCTCGAGAAGAGACCTCATTGAGCGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGCTCAATGAGGTCTCTTCTCGAGAAGAGACCTCATTGAGCGCAG-3'

Other clones with same target sequence:

(none)