Construct: shRNA TRCN0000114707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199022.1-1266s1c1
DNA Barcode:
GCGGTGTGTATAAGAACTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shc4 (271849)
Gene Description:
SHC (Src homology 2 domain containing) family, member 4
Transcript:
RefSeq NM_199022.1 (NON-CURRENT)
Match location:
Position 1266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTGTGTATAAGAACTGTA
Hairpin Sequence:
5'-CCGG-GCGGTGTGTATAAGAACTGTA-CTCGAG-TACAGTTCTTATACACACCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTGTGTATAAGAACTGTACTCGAGTACAGTTCTTATACACACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTGTGTATAAGAACTGTACTCGAGTACAGTTCTTATACACACCGC-3'

Other clones with same target sequence:

(none)