Construct: shRNA TRCN0000114821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011341.3-1741s1c1
DNA Barcode:
GCCCAGGTTTATTAAAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sdf4 (20318)
Gene Description:
stromal cell derived factor 4
Transcript:
RefSeq NM_011341.3 (NON-CURRENT)
Match location:
Position 1741 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGGTTTATTAAAGATAT
Hairpin Sequence:
5'-CCGG-GCCCAGGTTTATTAAAGATAT-CTCGAG-ATATCTTTAATAAACCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGGTTTATTAAAGATATCTCGAGATATCTTTAATAAACCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGGTTTATTAAAGATATCTCGAGATATCTTTAATAAACCTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000319869