Construct: shRNA TRCN0000114842

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025374.2-163s1c1
DNA Barcode:
GCAGCAAACGATGCTAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glo1 (109801)
Gene Description:
glyoxalase 1
Transcript:
RefSeq NM_025374.2 (NON-CURRENT)
Match location:
Position 163 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114842)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAAACGATGCTAAGAAT
Hairpin Sequence:
5'-CCGG-GCAGCAAACGATGCTAAGAAT-CTCGAG-ATTCTTAGCATCGTTTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAAACGATGCTAAGAATCTCGAGATTCTTAGCATCGTTTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAAACGATGCTAAGAATCTCGAGATTCTTAGCATCGTTTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000328458