Construct: shRNA TRCN0000114853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009665.2-1211s1c1
DNA Barcode:
CCCAGAAGATTGACGGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Amd1 (11702)
Gene Description:
S-adenosylmethionine decarboxylase 1
Transcript:
RefSeq NM_009665.2 (NON-CURRENT)
Match location:
Position 1211 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAGATTGACGGCTTTA
Hairpin Sequence:
5'-CCGG-CCCAGAAGATTGACGGCTTTA-CTCGAG-TAAAGCCGTCAATCTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAGATTGACGGCTTTACTCGAGTAAAGCCGTCAATCTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAGATTGACGGCTTTACTCGAGTAAAGCCGTCAATCTTCTGGG-3'

Other clones with same target sequence:

(none)