Construct: shRNA TRCN0000114901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172263.1-2478s1c1
DNA Barcode:
CCCAAACTTCATTTCCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pde8b (218461)
Gene Description:
phosphodiesterase 8B
Transcript:
RefSeq NM_172263.1 (NON-CURRENT)
Match location:
Position 2478 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAACTTCATTTCCAGAAA
Hairpin Sequence:
5'-CCGG-CCCAAACTTCATTTCCAGAAA-CTCGAG-TTTCTGGAAATGAAGTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAACTTCATTTCCAGAAACTCGAGTTTCTGGAAATGAAGTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAACTTCATTTCCAGAAACTCGAGTTTCTGGAAATGAAGTTTGGG-3'

Other clones with same target sequence:

(none)