Construct: shRNA TRCN0000114984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018779.1-3314s1c1
DNA Barcode:
CCAATCTTCAGGAATCCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pde3a (54611)
Gene Description:
phosphodiesterase 3A, cGMP inhibited
Transcript:
RefSeq NM_018779.1 (CURRENT)
Match location:
Position 3314 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCTTCAGGAATCCTTTA
Hairpin Sequence:
5'-CCGG-CCAATCTTCAGGAATCCTTTA-CTCGAG-TAAAGGATTCCTGAAGATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCTTCAGGAATCCTTTACTCGAGTAAAGGATTCCTGAAGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCTTCAGGAATCCTTTACTCGAGTAAAGGATTCCTGAAGATTGG-3'

Other clones with same target sequence:

(none)