Construct: shRNA TRCN0000114993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153422.1-1962s1c1
DNA Barcode:
GCGTTGCTCATTGCTGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pde5a (242202)
Gene Description:
phosphodiesterase 5A, cGMP-specific
Transcript:
RefSeq NM_153422.1 (NON-CURRENT)
Match location:
Position 1962 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000114993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTGCTCATTGCTGCTTTA
Hairpin Sequence:
5'-CCGG-GCGTTGCTCATTGCTGCTTTA-CTCGAG-TAAAGCAGCAATGAGCAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTGCTCATTGCTGCTTTACTCGAGTAAAGCAGCAATGAGCAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTGCTCATTGCTGCTTTACTCGAGTAAAGCAGCAATGAGCAACGC-3'

Other clones with same target sequence:

(none)