Construct: shRNA TRCN0000115002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008800.1-1485s1c1
DNA Barcode:
CCTTCTTTAGATGTGGACGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pde1b (18574)
Gene Description:
phosphodiesterase 1B, Ca2+-calmodulin dependent
Transcript:
RefSeq NM_008800.1 (NON-CURRENT)
Match location:
Position 1485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTTTAGATGTGGACGTA
Hairpin Sequence:
5'-CCGG-CCTTCTTTAGATGTGGACGTA-CTCGAG-TACGTCCACATCTAAAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTTTAGATGTGGACGTACTCGAGTACGTCCACATCTAAAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTTTAGATGTGGACGTACTCGAGTACGTCCACATCTAAAGAAGG-3'

Other clones with same target sequence:

(none)