Construct: shRNA TRCN0000115074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133704.3-163s1c1
DNA Barcode:
GCTGTCTGCATCCACTGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec22a (317717)
Gene Description:
SEC22 homolog A, vesicle trafficking protein
Transcript:
RefSeq NM_133704.3 (NON-CURRENT)
Match location:
Position 163 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCTGCATCCACTGATTA
Hairpin Sequence:
5'-CCGG-GCTGTCTGCATCCACTGATTA-CTCGAG-TAATCAGTGGATGCAGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCTGCATCCACTGATTACTCGAGTAATCAGTGGATGCAGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCTGCATCCACTGATTACTCGAGTAATCAGTGGATGCAGACAGC-3'

Other clones with same target sequence:

(none)