Construct: shRNA TRCN0000115103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009946.1-433s1c1
DNA Barcode:
CTGGACACAGTGCTCAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cplx2 (12890)
Gene Description:
complexin 2
Transcript:
RefSeq NM_009946.1 (NON-CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGACACAGTGCTCAAATAT
Hairpin Sequence:
5'-CCGG-CTGGACACAGTGCTCAAATAT-CTCGAG-ATATTTGAGCACTGTGTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGACACAGTGCTCAAATATCTCGAGATATTTGAGCACTGTGTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGACACAGTGCTCAAATATCTCGAGATATTTGAGCACTGTGTCCAG-3'

Other clones with same target sequence:

(none)