Construct: shRNA TRCN0000115112

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025812.2-741s1c1
DNA Barcode:
CCCTATATTTACCGAAGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmg20a (66867)
Gene Description:
high mobility group 20A
Transcript:
RefSeq NM_025812.2 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115112)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATATTTACCGAAGAATT
Hairpin Sequence:
5'-CCGG-CCCTATATTTACCGAAGAATT-CTCGAG-AATTCTTCGGTAAATATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATATTTACCGAAGAATTCTCGAGAATTCTTCGGTAAATATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATATTTACCGAAGAATTCTCGAGAATTCTTCGGTAAATATAGGG-3'

Other clones with same target sequence:

(none)