Construct: shRNA TRCN0000115125

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010727.2-719s1c1
DNA Barcode:
CCAAGGTTGTTTCACCTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lnx1 (16924)
Gene Description:
ligand of numb-protein X 1
Transcript:
RefSeq NM_010727.2 (NON-CURRENT)
Match location:
Position 719 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115125)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGTTGTTTCACCTGATT
Hairpin Sequence:
5'-CCGG-CCAAGGTTGTTTCACCTGATT-CTCGAG-AATCAGGTGAAACAACCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGTTGTTTCACCTGATTCTCGAGAATCAGGTGAAACAACCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGTTGTTTCACCTGATTCTCGAGAATCAGGTGAAACAACCTTGG-3'

Other clones with same target sequence:

(none)