Construct: shRNA TRCN0000115172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145392.1-638s1c1
DNA Barcode:
GCCATTAAACTCCTAGAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bag2 (213539)
Gene Description:
BCL2-associated athanogene 2
Transcript:
RefSeq NM_145392.1 (NON-CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTAAACTCCTAGAGCAT
Hairpin Sequence:
5'-CCGG-GCCATTAAACTCCTAGAGCAT-CTCGAG-ATGCTCTAGGAGTTTAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTAAACTCCTAGAGCATCTCGAGATGCTCTAGGAGTTTAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTAAACTCCTAGAGCATCTCGAGATGCTCTAGGAGTTTAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000325333