Construct: shRNA TRCN0000115218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030730.1-2934s1c1
DNA Barcode:
CCCAAACATCTTTGGATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad54l2 (81000)
Gene Description:
RAD54 like 2 (S. cerevisiae)
Transcript:
RefSeq NM_030730.1 (NON-CURRENT)
Match location:
Position 2934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAACATCTTTGGATATAA
Hairpin Sequence:
5'-CCGG-CCCAAACATCTTTGGATATAA-CTCGAG-TTATATCCAAAGATGTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAACATCTTTGGATATAACTCGAGTTATATCCAAAGATGTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAACATCTTTGGATATAACTCGAGTTATATCCAAAGATGTTTGGG-3'

Other clones with same target sequence:

(none)