Construct: shRNA TRCN0000115341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018748.2-7335s1c1
DNA Barcode:
CCTAGTTCTTTATGCTATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Golga4 (54214)
Gene Description:
golgi autoantigen, golgin subfamily a, 4
Transcript:
RefSeq NM_018748.2 (NON-CURRENT)
Match location:
Position 7335 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGTTCTTTATGCTATGAT
Hairpin Sequence:
5'-CCGG-CCTAGTTCTTTATGCTATGAT-CTCGAG-ATCATAGCATAAAGAACTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGTTCTTTATGCTATGATCTCGAGATCATAGCATAAAGAACTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGTTCTTTATGCTATGATCTCGAGATCATAGCATAAAGAACTAGG-3'

Other clones with same target sequence:

  1. TRCN0000326616