Construct: shRNA TRCN0000115374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009677.3-2648s1c1
DNA Barcode:
CGGATCAAGCTCACATATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap1g1 (11765)
Gene Description:
adaptor protein complex AP-1, gamma 1 subunit
Transcript:
RefSeq NM_009677.3 (NON-CURRENT)
Match location:
Position 2648 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATCAAGCTCACATATAAT
Hairpin Sequence:
5'-CCGG-CGGATCAAGCTCACATATAAT-CTCGAG-ATTATATGTGAGCTTGATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATCAAGCTCACATATAATCTCGAGATTATATGTGAGCTTGATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATCAAGCTCACATATAATCTCGAGATTATATGTGAGCTTGATCCG-3'

Other clones with same target sequence:

(none)