Construct: shRNA TRCN0000115425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004146.1-496s1c1
DNA Barcode:
CGCCTCAACCAAGTAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pisd-ps1 (236604)
Gene Description:
phosphatidylserine decarboxylase, pseudogene 1
Transcript:
RefSeq NM_001004146.1 (NON-CURRENT)
Match location:
Position 496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTCAACCAAGTAGAACTT
Hairpin Sequence:
5'-CCGG-CGCCTCAACCAAGTAGAACTT-CTCGAG-AAGTTCTACTTGGTTGAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTCAACCAAGTAGAACTTCTCGAGAAGTTCTACTTGGTTGAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTCAACCAAGTAGAACTTCTCGAGAAGTTCTACTTGGTTGAGGCG-3'

Other clones with same target sequence:

(none)