Construct: shRNA TRCN0000115431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013888.1-873s1c1
DNA Barcode:
GCTCTGTATCATTGATTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc12 (30045)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C12
Transcript:
RefSeq NM_013888.1 (NON-CURRENT)
Match location:
Position 873 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGTATCATTGATTCTTA
Hairpin Sequence:
5'-CCGG-GCTCTGTATCATTGATTCTTA-CTCGAG-TAAGAATCAATGATACAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGTATCATTGATTCTTACTCGAGTAAGAATCAATGATACAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGTATCATTGATTCTTACTCGAGTAAGAATCAATGATACAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000318128