Construct: shRNA TRCN0000115560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194343.1-371s1c1
DNA Barcode:
GCAAGACTCATTGTCCTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim45 (229644)
Gene Description:
tripartite motif-containing 45
Transcript:
RefSeq NM_194343.1 (NON-CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGACTCATTGTCCTACAT
Hairpin Sequence:
5'-CCGG-GCAAGACTCATTGTCCTACAT-CTCGAG-ATGTAGGACAATGAGTCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGACTCATTGTCCTACATCTCGAGATGTAGGACAATGAGTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGACTCATTGTCCTACATCTCGAGATGTAGGACAATGAGTCTTGC-3'

Other clones with same target sequence:

(none)