Construct: shRNA TRCN0000115684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014865.2-3775s1c1
DNA Barcode:
CCTCCGTAAGATGCTTGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCAPD2 (9918)
Gene Description:
non-SMC condensin I complex subunit D2
Transcript:
RefSeq NM_014865.2 (NON-CURRENT)
Match location:
Position 3775 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCGTAAGATGCTTGACAA
Hairpin Sequence:
5'-CCGG-CCTCCGTAAGATGCTTGACAA-CTCGAG-TTGTCAAGCATCTTACGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCGTAAGATGCTTGACAACTCGAGTTGTCAAGCATCTTACGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCGTAAGATGCTTGACAACTCGAGTTGTCAAGCATCTTACGGAGG-3'

Other clones with same target sequence:

(none)