Construct: shRNA TRCN0000115744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016383.2-332s1c1
DNA Barcode:
GCCTTTAATTGAGCAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LUZP4 (51213)
Gene Description:
leucine zipper protein 4
Transcript:
RefSeq NM_016383.2 (NON-CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTAATTGAGCAGGAGAA
Hairpin Sequence:
5'-CCGG-GCCTTTAATTGAGCAGGAGAA-CTCGAG-TTCTCCTGCTCAATTAAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTAATTGAGCAGGAGAACTCGAGTTCTCCTGCTCAATTAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTAATTGAGCAGGAGAACTCGAGTTCTCCTGCTCAATTAAAGGC-3'

Other clones with same target sequence:

(none)