Construct: shRNA TRCN0000115813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004909.1-225s1c1
DNA Barcode:
CTACCACAAGACTAACATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSAG2 (9598)
Gene Description:
CSAG family, member 2
Transcript:
RefSeq NM_004909.1 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCACAAGACTAACATAAA
Hairpin Sequence:
5'-CCGG-CTACCACAAGACTAACATAAA-CTCGAG-TTTATGTTAGTCTTGTGGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCACAAGACTAACATAAACTCGAGTTTATGTTAGTCTTGTGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCACAAGACTAACATAAACTCGAGTTTATGTTAGTCTTGTGGTAG-3'

Other clones with same target sequence:

(none)