Construct: shRNA TRCN0000115881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024674.1-386s1c1
DNA Barcode:
GAGTCTCACCACGGCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIN52 (91750)
Gene Description:
lin-52 DREAM MuvB core complex component
Transcript:
RefSeq NM_001024674.1 (NON-CURRENT)
Match location:
Position 386 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCTCACCACGGCTAATTT
Hairpin Sequence:
5'-CCGG-GAGTCTCACCACGGCTAATTT-CTCGAG-AAATTAGCCGTGGTGAGACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCTCACCACGGCTAATTTCTCGAGAAATTAGCCGTGGTGAGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCTCACCACGGCTAATTTCTCGAGAAATTAGCCGTGGTGAGACTC-3'

Other clones with same target sequence:

  1. TRCN0000286168