Construct: shRNA TRCN0000115886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152729.2-1343s1c1
DNA Barcode:
CGCAGAATTACCTCTGGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NT5DC1 (221294)
Gene Description:
5'-nucleotidase domain containing 1
Transcript:
RefSeq NM_152729.2 (NON-CURRENT)
Match location:
Position 1343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGAATTACCTCTGGACTA
Hairpin Sequence:
5'-CCGG-CGCAGAATTACCTCTGGACTA-CTCGAG-TAGTCCAGAGGTAATTCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGAATTACCTCTGGACTACTCGAGTAGTCCAGAGGTAATTCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGAATTACCTCTGGACTACTCGAGTAGTCCAGAGGTAATTCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000292075