Construct: shRNA TRCN0000115936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014051.2-362s1c1
DNA Barcode:
GAGATTTAAGAGGTCCAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM14A (28978)
Gene Description:
transmembrane protein 14A
Transcript:
RefSeq NM_014051.2 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATTTAAGAGGTCCAAGAA
Hairpin Sequence:
5'-CCGG-GAGATTTAAGAGGTCCAAGAA-CTCGAG-TTCTTGGACCTCTTAAATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATTTAAGAGGTCCAAGAACTCGAGTTCTTGGACCTCTTAAATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATTTAAGAGGTCCAAGAACTCGAGTTCTTGGACCTCTTAAATCTC-3'

Other clones with same target sequence:

  1. TRCN0000291158