Construct: shRNA TRCN0000115957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014888.1-1767s1c1
DNA Barcode:
CCTGTGTTTATCTAACTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM3C (10447)
Gene Description:
FAM3 metabolism regulating signaling molecule C
Transcript:
RefSeq NM_014888.1 (NON-CURRENT)
Match location:
Position 1767 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTTTATCTAACTTCAT
Hairpin Sequence:
5'-CCGG-CCTGTGTTTATCTAACTTCAT-CTCGAG-ATGAAGTTAGATAAACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTTTATCTAACTTCATCTCGAGATGAAGTTAGATAAACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTTTATCTAACTTCATCTCGAGATGAAGTTAGATAAACACAGG-3'

Other clones with same target sequence:

(none)