Construct: shRNA TRCN0000115960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014888.1-328s1c1
DNA Barcode:
CCAGATATAAGTGTGGGATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM3C (10447)
Gene Description:
FAM3 metabolism regulating signaling molecule C
Transcript:
RefSeq NM_014888.1 (NON-CURRENT)
Match location:
Position 328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000115960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATATAAGTGTGGGATCT
Hairpin Sequence:
5'-CCGG-CCAGATATAAGTGTGGGATCT-CTCGAG-AGATCCCACACTTATATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATATAAGTGTGGGATCTCTCGAGAGATCCCACACTTATATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATATAAGTGTGGGATCTCTCGAGAGATCCCACACTTATATCTGG-3'

Other clones with same target sequence:

(none)