Construct: shRNA TRCN0000116050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001425.1-371s1c1
DNA Barcode:
ACGTGGAACAACGACACCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EMP3 (2014)
Gene Description:
epithelial membrane protein 3
Transcript:
RefSeq NM_001425.1 (NON-CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGGAACAACGACACCAAA
Hairpin Sequence:
5'-CCGG-ACGTGGAACAACGACACCAAA-CTCGAG-TTTGGTGTCGTTGTTCCACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGGAACAACGACACCAAACTCGAGTTTGGTGTCGTTGTTCCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGGAACAACGACACCAAACTCGAGTTTGGTGTCGTTGTTCCACGT-3'

Other clones with same target sequence:

(none)