Construct: shRNA TRCN0000116097

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016848.2-2222s1c1
DNA Barcode:
GCTTCCTTTAAGGTCAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHC3 (53358)
Gene Description:
SHC adaptor protein 3
Transcript:
RefSeq NM_016848.2 (NON-CURRENT)
Match location:
Position 2222 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116097)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTTTAAGGTCAAGTTT
Hairpin Sequence:
5'-CCGG-GCTTCCTTTAAGGTCAAGTTT-CTCGAG-AAACTTGACCTTAAAGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTTTAAGGTCAAGTTTCTCGAGAAACTTGACCTTAAAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTTTAAGGTCAAGTTTCTCGAGAAACTTGACCTTAAAGGAAGC-3'

Other clones with same target sequence:

(none)