Construct: shRNA TRCN0000116104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018303.4-1915s1c1
DNA Barcode:
CGGCGGAAGAAATAAAGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOC2 (55770)
Gene Description:
exocyst complex component 2
Transcript:
RefSeq NM_018303.4 (NON-CURRENT)
Match location:
Position 1915 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCGGAAGAAATAAAGAGAT
Hairpin Sequence:
5'-CCGG-CGGCGGAAGAAATAAAGAGAT-CTCGAG-ATCTCTTTATTTCTTCCGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCGGAAGAAATAAAGAGATCTCGAGATCTCTTTATTTCTTCCGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCGGAAGAAATAAAGAGATCTCGAGATCTCTTTATTTCTTCCGCCG-3'

Other clones with same target sequence:

  1. TRCN0000289959