Construct: shRNA TRCN0000116146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015589.3-211s1c1
DNA Barcode:
GCAACAGGAATCCAAGGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMD4A (23034)
Gene Description:
sterile alpha motif domain containing 4A
Transcript:
RefSeq NM_015589.3 (NON-CURRENT)
Match location:
Position 211 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAGGAATCCAAGGATAA
Hairpin Sequence:
5'-CCGG-GCAACAGGAATCCAAGGATAA-CTCGAG-TTATCCTTGGATTCCTGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAGGAATCCAAGGATAACTCGAGTTATCCTTGGATTCCTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAGGAATCCAAGGATAACTCGAGTTATCCTTGGATTCCTGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000288937