Construct: shRNA TRCN0000116149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012223.2-1999s1c1
DNA Barcode:
CCCAAACTATATTAGGTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO1B (4430)
Gene Description:
myosin IB
Transcript:
RefSeq NM_012223.2 (NON-CURRENT)
Match location:
Position 1999 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAACTATATTAGGTGTAT
Hairpin Sequence:
5'-CCGG-CCCAAACTATATTAGGTGTAT-CTCGAG-ATACACCTAATATAGTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAACTATATTAGGTGTATCTCGAGATACACCTAATATAGTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAACTATATTAGGTGTATCTCGAGATACACCTAATATAGTTTGGG-3'

Other clones with same target sequence:

(none)